RT by @Plants_EFSA: Behind the scenes, indentify #2 : @aimethods DNA Barcoding.

Black-headed leafcutter bee, Megachile circumcincta. The DNA was isolated from a leg of the bee using a liquid (lysis buffer). Means everything dissolves in it except for the chitin. Then the Co1 gene was amplified with PCR. This means that certain parts of the DNA are copied so often that they are easily detectable. Detectable for the sequencer, the next step. The 313 base pairs required for the identification are then determined here. (See colorful graphic) With the help of a worldwide database of already certain animals whose Co1 gene has been determined, the base pairs can be compared with each other.

#dnabarcoding #barcoding #dna #macro #insects #bumblebee #hummel #insekten

🐦🔗: https://nitter.cz/sagaOptics/status/1725182377717108888#m

[2023-11-16 16:01 UTC]